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  • Ning Zhang

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Project Title2011-133 Dimeric Diagnostic DNA Arrays for Pathogen Detection and Identification
Track Code2011-133
Websitehttp://otc.rutgers.edu/
Short Description

Rutgers scientists have developed a unique and pioneering technology for detection and identification of important pathogens in the form of a macroarray diagnostic technology with enhanced detection sensitivity and small reduction in specificity using repeat sequence probes (dimers). We continue to have positive results and improvements, and believe the next stage will focus on refinement, development of a portable ‘kit’, and pathogen quantification, among others.

Abstract

Rutgers scientists have developed a unique and pioneering technology for detection and identification of  important pathogens in the form of a macroarray diagnostic technology with enhanced detection sensitivity and small reduction in specificity using repeat sequence probes (dimers). We continue to have positive results and improvements, and believe the next stage will focus on refinement, development of a portable ‘kit’, and pathogen quantification, among others.

To date, Dr. Zhang has produced membrane-based macroarrays that include five types of oligonucleotide probes. The probes were designed based on the internal transcribed spacer sequences of rRNA genes of four important microbial pathogens of cereals, turfgrass, and other plants, i.e., Rhizoctonia solani, Pythium aphanidermatum, Fusarium solani, and F. oxysporum, to compare the performance of the probes. The five probes studied for each type of strains were monomers (M; 20-24nt), monomers with a 10-adenine tail (M-A10), monomers with a 20-adenine tail (M-A20), dimers (D; 40-48nt), and dimers with a 10-adenine spacer (DA). Among all probes, D and DA probes displayed significantly higher sensitivity than the M, M-A10, and M-A12 probes. The dimeric probes D also had lower signal variability, thereby increasing the macroarray signal uniformity. The developed method is rapid and can be useful for early human, animal, or plant diagnosis, as well as for water and environmental analysis.  Numerous additional probes are in the process of being designed and tested as part of this continuing program.

 
TagsPathogen Detection; Human, animal, and Plant (agricultural crops) Diagnostic; Water and Environmental Analysis.
 
Posted DateMay 7, 2012 2:51 PM

Researcher

Name
Ning Zhang

Manager

Name
Leon Segal

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